Coding

Part:BBa_K5335034:Design

Designed by: Wentao Yang   Group: iGEM24_HZAU-China   (2024-10-02)


A cluster of 2 genes that generate and transport TAA to kill nematodes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1793
    Illegal AgeI site found at 1569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the original sequence without Condon optimization


Source

Bacillus thuringiensis

References